Constitutive over-expression of pectinases in Penicillium occitanis CT1 mutant is transcriptionally regulated.
Identifieur interne : 000118 ( Main/Exploration ); précédent : 000117; suivant : 000119Constitutive over-expression of pectinases in Penicillium occitanis CT1 mutant is transcriptionally regulated.
Auteurs : Malika Ayadi [Tunisie] ; Sameh Trigui ; Hèla Trigui-Lahiani ; Noomen Hadj-Taïeb ; Mohamed Jaoua ; Ali GargouriSource :
- Biotechnology letters [ 1573-6776 ] ; 2011.
Descripteurs français
- KwdFr :
- ARN fongique (génétique), ARN fongique (métabolisme), ARN messager (génétique), ARN messager (métabolisme), Biotechnologie (MeSH), Données de séquences moléculaires (MeSH), Facteurs de transcription (génétique), Facteurs de transcription (métabolisme), Gènes fongiques (MeSH), Mutation (MeSH), Penicillium (croissance et développement), Penicillium (enzymologie), Penicillium (génétique), Polygalacturonase (génétique), Polygalacturonase (métabolisme), Protéines fongiques (génétique), Protéines fongiques (métabolisme), Régulation de l'expression des gènes codant pour des enzymes (MeSH), Régulation de l'expression des gènes fongiques (MeSH), Similitude de séquences d'acides aminés (MeSH), Séquence d'acides aminés (MeSH), Séquence nucléotidique (MeSH).
- MESH :
- croissance et développement : Penicillium.
- enzymologie : Penicillium.
- génétique : ARN fongique, ARN messager, Facteurs de transcription, Penicillium, Polygalacturonase, Protéines fongiques.
- métabolisme : ARN fongique, ARN messager, Facteurs de transcription, Polygalacturonase, Protéines fongiques.
- Biotechnologie, Données de séquences moléculaires, Gènes fongiques, Mutation, Régulation de l'expression des gènes codant pour des enzymes, Régulation de l'expression des gènes fongiques, Similitude de séquences d'acides aminés, Séquence d'acides aminés, Séquence nucléotidique.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Base Sequence (MeSH), Biotechnology (MeSH), Fungal Proteins (genetics), Fungal Proteins (metabolism), Gene Expression Regulation, Enzymologic (MeSH), Gene Expression Regulation, Fungal (MeSH), Genes, Fungal (MeSH), Molecular Sequence Data (MeSH), Mutation (MeSH), Penicillium (enzymology), Penicillium (genetics), Penicillium (growth & development), Polygalacturonase (genetics), Polygalacturonase (metabolism), RNA, Fungal (genetics), RNA, Fungal (metabolism), RNA, Messenger (genetics), RNA, Messenger (metabolism), Sequence Homology, Amino Acid (MeSH), Transcription Factors (genetics), Transcription Factors (metabolism).
- MESH :
- chemical , genetics : Fungal Proteins, Polygalacturonase, RNA, Fungal, RNA, Messenger, Transcription Factors.
- chemical , metabolism : Fungal Proteins, Polygalacturonase, RNA, Fungal, RNA, Messenger, Transcription Factors.
- enzymology : Penicillium.
- genetics : Penicillium.
- growth & development : Penicillium.
- Amino Acid Sequence, Base Sequence, Biotechnology, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Fungal, Genes, Fungal, Molecular Sequence Data, Mutation, Sequence Homology, Amino Acid.
Abstract
The CT1 mutant of Penicillium occitanis hyperproduces extracellular pectinases constitutively since it secretes pectinases even on glucose-containing medium. We show here that all other hydrolytic enzymes remain at low activities in CT1, confirming the specificity of the regulatory mutation towards pectinases. We isolated, by RT-PCR and through the construction of a cDNA library, three fragments coding for: a pectin lyase (pnl1), a polygalacturonase (pga1) and a pectate lyase (pal1). These fragments were used as probes in Northern blots analysis of the wild type strain CL100 and the CT1 mutant of P. occitanis grown in three culture conditions. The CT1 mutant showed a very high amount of pnl1, pga1 and pal1 mRNA either in pectin, glucose or glycerol grown cells while in the wild type CL100 strain, all transcripts were undetectable even on pectin. These results suggest that the CT1 mutation affects a trans-regulatory transcriptional factor regulating pectinase expression.
DOI: 10.1007/s10529-011-0546-3
PubMed: 21287229
Affiliations:
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Le document en format XML
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Box 1177, 3018, Sfax</wicri:regionArea><wicri:noRegion>Sfax</wicri:noRegion></affiliation></author><author><name sortKey="Trigui, Sameh" sort="Trigui, Sameh" uniqKey="Trigui S" first="Sameh" last="Trigui">Sameh Trigui</name></author><author><name sortKey="Trigui Lahiani, Hela" sort="Trigui Lahiani, Hela" uniqKey="Trigui Lahiani H" first="Hèla" last="Trigui-Lahiani">Hèla Trigui-Lahiani</name></author><author><name sortKey="Hadj Taieb, Noomen" sort="Hadj Taieb, Noomen" uniqKey="Hadj Taieb N" first="Noomen" last="Hadj-Taïeb">Noomen Hadj-Taïeb</name></author><author><name sortKey="Jaoua, Mohamed" sort="Jaoua, Mohamed" uniqKey="Jaoua M" first="Mohamed" last="Jaoua">Mohamed Jaoua</name></author><author><name sortKey="Gargouri, Ali" sort="Gargouri, Ali" uniqKey="Gargouri A" first="Ali" last="Gargouri">Ali Gargouri</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21287229</idno><idno type="pmid">21287229</idno><idno type="doi">10.1007/s10529-011-0546-3</idno><idno type="wicri:Area/PubMed/Corpus">000699</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000699</idno><idno type="wicri:Area/PubMed/Curation">000695</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000695</idno><idno type="wicri:Area/PubMed/Checkpoint">000692</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000692</idno><idno type="wicri:Area/Main/Merge">000118</idno><idno type="wicri:Area/Main/Curation">000118</idno><idno type="wicri:Area/Main/Exploration">000118</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Constitutive over-expression of pectinases in Penicillium occitanis CT1 mutant is transcriptionally regulated.</title><author><name sortKey="Ayadi, Malika" sort="Ayadi, Malika" uniqKey="Ayadi M" first="Malika" last="Ayadi">Malika Ayadi</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Biomass Valorisation and Production of Proteins in Eukaryotes, Centre of Biotechnology of Sfax, University of Sfax, Route Sidi Mansour Km 6, P. 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de séquences moléculaires</term><term>Gènes fongiques</term><term>Mutation</term><term>Régulation de l'expression des gènes codant pour des enzymes</term><term>Régulation de l'expression des gènes fongiques</term><term>Similitude de séquences d'acides aminés</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The CT1 mutant of Penicillium occitanis hyperproduces extracellular pectinases constitutively since it secretes pectinases even on glucose-containing medium. We show here that all other hydrolytic enzymes remain at low activities in CT1, confirming the specificity of the regulatory mutation towards pectinases. We isolated, by RT-PCR and through the construction of a cDNA library, three fragments coding for: a pectin lyase (pnl1), a polygalacturonase (pga1) and a pectate lyase (pal1). These fragments were used as probes in Northern blots analysis of the wild type strain CL100 and the CT1 mutant of P. occitanis grown in three culture conditions. The CT1 mutant showed a very high amount of pnl1, pga1 and pal1 mRNA either in pectin, glucose or glycerol grown cells while in the wild type CL100 strain, all transcripts were undetectable even on pectin. These results suggest that the CT1 mutation affects a trans-regulatory transcriptional factor regulating pectinase expression.</div></front></TEI><affiliations><list><country><li>Tunisie</li></country></list><tree><noCountry><name sortKey="Gargouri, Ali" sort="Gargouri, Ali" uniqKey="Gargouri A" first="Ali" last="Gargouri">Ali Gargouri</name><name sortKey="Hadj Taieb, Noomen" sort="Hadj Taieb, Noomen" uniqKey="Hadj Taieb N" first="Noomen" last="Hadj-Taïeb">Noomen Hadj-Taïeb</name><name sortKey="Jaoua, Mohamed" sort="Jaoua, Mohamed" uniqKey="Jaoua M" first="Mohamed" last="Jaoua">Mohamed Jaoua</name><name sortKey="Trigui Lahiani, Hela" sort="Trigui Lahiani, Hela" uniqKey="Trigui Lahiani H" first="Hèla" last="Trigui-Lahiani">Hèla Trigui-Lahiani</name><name sortKey="Trigui, Sameh" sort="Trigui, Sameh" uniqKey="Trigui S" first="Sameh" last="Trigui">Sameh Trigui</name></noCountry><country name="Tunisie"><noRegion><name sortKey="Ayadi, Malika" sort="Ayadi, Malika" uniqKey="Ayadi M" first="Malika" last="Ayadi">Malika Ayadi</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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